Protocols

Marclab Protocols

Marc Lab Guide 4.1 Winter 2002 [Adobe® PDF]

NUMBERS

FORMS

COLOR TABLES

  • CellKit Theme Map Color Table for Adobe Photoshop: idl.act (right-click & save)
  • CellKit Theme Map Color Table for PCI Geomatica: idlpct.pix (right-click & save)
  • CellKit Slicer Color Table for greyscale indexing in Photoshop: cym_slicer_white.act (right-click & save)

SYNCROSCAN CAPTURE RULES

FOLDER NAMING RULES (see complete rules for details)

  • All folders are kept in the folder image_archive
  • All folder names are 5 numerics (slide number) + date (yymmdd): NNNNN_yymmdd
  • Folder 03665_040422 = all images from slide 3665 taken 22 April 2004
  • You may duplicate files into personal folders (e.g. RM or WillieNelson)

FILE NAMING RULES (see complete rules for details)

  • Filename = NNNNN_iii_r###_s##_t##_ppp_mmm.tif
  • NNNNN = 5 numerics slide number (example: 03665)
  • iii = your initals (example: rem)
  • r### = resolution in nm/pix (example r365, see IMAGE CAPTURE SCALES)
  • s## = spot number (example: s01 through s12 for HTC slides)
  • t## = set number (example: t03 is the third non-montage region captured)
  • ppp = alphabetic probe code (example: yy GABA; use zz if the code is not on the list, request a code)
  • mmm = alphabetic montage code (example: c = third montage element in a series)

IMAGE CAPTURE SCALES

Leica Syncroscan 01 Zeiss

 

Mag nm/pix pix/µm Mag nm/pix pix/µm
10x 725 1.38 1x 6490 0.15
20x 365 2.74 4x 1870 0.53
40x 182 5.50 10x 890 1.12
63x 117 8.58 16x 543 1.84
100x 73 13.64 25x 345 2.90
25x oil 350 2.86
40x oil 221 4.52
63x oil 142 7.06
100x oil 88 11.3

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